Exploring Q5 high GC enhancer – High-Fidelity DNA Polymerase, Q5 High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance.
About the product
One of the finest products of New England Biolabs (NEB), is a recognized world leader in the discovery, development, and commercialization of reagents for genomic research.
Q5 High-fidelity DNA Polymerase sets a new standard both for reliability and robust performance. Q5 DNA Polymerase results in extremely low error rates with a maximum fidelity amplification (~280 times higher than Taq). Q5 DNA Polymerase consists of a novel polymerase, fused with the Sso7d DNA binding domain for increased progressiveness, increasing speed, trustworthiness and performance reliability.
- Highest fidelity amplification (~280X higher than Taq)
- Ultra-low error rates
- Superior performance for a broad range of amplicons (from high AT to high GC)
- Hot start and master mix formats available
Q5 High-Fidelity DNA polymerase is an extremely thermo-fidel, 3 “to 5” DNA polymerase, combined to support robust DNA amplification in a processing Sso7d domain. Q5 High-Fidelity DNA Polymerase is perfect for cloning and can be used with long or difficult amplicons, with an error rate of < 280 times lower than that with Taq DNA Polymerase.
Q5 High-fidelity DNA Polymerase comes with a buffer system that provides robust amplification irrespective of the content of GC. The 5X Q5 Reaction Buffer contains 2 mM Mg++ in final (1x) responses and is advisable for most routine applications. Amplification can be improved by adding a 5X Q5 High GC Enhancer for GC-rich targets (by around 65 percent GC). Q5 DNA Polymerases of high fidelity are unlike typical PCR enzymes or less fidelity. The use of the NEB Tm Calculator is highly recommended to determine optimal rinsing temperatures for a certain set of primers.
- High-fidelity PCR
- Long or Difficult Amplification
- High-throughput PCR
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.
Unit Assay Conditions
25 mM TAPS-HCl (pH 9.3 @ 25°C), 50 mM KCl, 2 mM MgCl2, 1 mM β-mercaptoethanol, 200 μM dNTPs including [3H]-dTTP and 15 nM primed M13 DNA.
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